Coding

Part:BBa_K512002:Design

Designed by: Dongzhu Wu   Group: iGEM11_USC   (2011-09-28)


K12 CRISPR with GFP target spacer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 222
    Illegal BamHI site found at 344
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 222
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to be written


Source

K12

References